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Transcriptome Analysis Reveals a Gene Expression Pattern That Contributes to Sugarcane Bud Propagation Induced by Indole-3-Butyric Acid

文献类型： 外文期刊

作者： Xu, Lin 1 ; Deng, Zhi-Nian 1 ; Wu, Kai-Chao 1 ; Malviya, Mukesh Kumar 1 ; Solanki, Manoj Kumar 2 ; Verma, Krishan K. 1 ; Pang, Tian 1 ; Li, Yi-Jie 1 ; Liu, Xiao-Yan 1 ; Kashyap, Brijendra Kumar 3 ; Dessoky, Eldessoky S. 4 ; Wang, Wei-Zan 1 ; Huang, Hai-Rong 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Key Lab Sugarcane Biotechnol & Genet Improvement, Minist Agr & Rural Area,Sugarcane Res Ctr,Sugarca, Chinese Acad Agr Sci,Guangxi Key Lab Sugarcane Ge, Nanning, Peoples R China

2.Univ Silesia Katowice, Plant Cytogenet & Mol Biol Grp, Inst Biol Biotechnol & Environm Protect, Fac Nat Sci, Katowice, Poland

3.Bundelkhand Univ, Inst Engn & Technol, Dept Biotechnol Engn, Jhansi, Uttar Pradesh, India

4.Agr Res Ctr, Agr Genet Engn Res Inst, Dept Plant Genet Transformat, Giza, Egypt

5.Taif Univ, Coll Sci, Dept Biol, Taif, Saudi Arabia

关键词： IBA; single-bud seed; sugarcane; root; transcriptome

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： Sugarcane is a cash crop that plays an integral part in the sugar industry. The Sustainable Sugarcane Initiative (SSI) has been adopted globally, ensuring enough and aiming for more yield, helping increase disease-free sugarcane cultivation. Single-bud seeds could be the best approach for sugarcane cultivation. Indole-3-butyric acid (IBA) is a rooting agent utilized significantly in seedling propagation. Greenhouse experiment results discovered the significant growth promotion in sugarcane seedlings and accumulation of plant hormones at 100 ppm IBA. Next, we performed transcriptomic analysis of sugarcane buds using RNA sequencing and compared their gene expression during root development due to affect of IBA (100 ppm). A total of 113,475 unigenes were annotated with an average length of 836 by (N50= 1,536). The comparative RNA-seq study between the control (CK) and IBA-treated (T) buds showed significant differentially expressed unigenes (494 upregulated and 2086 downregulated). The IBA influenced major biological processes including metabolic process, the cellular process, and single-organism process. For cellular component category, cell, cell part, organelle, membrane, and organelle part were mainly affected. In addition, catalytic activity and binding were primarily affected in the molecular function categories. Furthermore, the expression of genes related to plant hormones and signaling pathways was analyzed by qRT-PCR, which was consistent with the RNA-seq expression profile. This study provides new insights into the IBA response to the bud sprouting in sugarcane based on RNA sequencing, and generated information could help further research on breeding improvement of sugarcane.

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