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EXPRESSIONS OF THREE MEMBERS OF ACC SYNTHASE GENE IN SUGARCANE STALKS INDUCED BY ETHEPHON IN RELATION TO SUGARACCUMULATION

文献类型： 会议论文

第一作者： AI-QIN WANG

作者： AI-QIN WANG 1 ; YE-GENG FAN; XIAO-YAN ZHAO; LONG-FEI HE; LI-TAO YANG; YANG-RUILI;

作者机构： 1.Biotechnology key Laboratory, Guangxi Academy of Agricultural Sciences, Manning 530007, China

关键词： Saccharum offtcinarum L. Hybrid;1-aminocyclopropane-1-carboxylic acid synthase;expression;sucrose accumulation

会议名称： Technologies to improve sugar productivity in developing countries

主办单位：

页码： 598-602

摘要： Aim: To study expressions of ACC synthese genes in stalks regulated with ethephon and its correlation with sucrose accumulation in sugarcane. Methods and results: Based on the cloning of Sc-ACS1, Sc-ACS2 and Sc-ACS3 of ACS gene families, their expressions were tested in the stalks of sugarcane with Sc-ACS1, Sc-ACS2 and Sc-ACS3 as the probes, respectively. The results of Northern blotting analysis showed that the expression of Sc-ACS1 in the mature internodes was higher than in the immature internodesbefore full maturation but on the contrary after full maturation, the expressions of Sc-ACS2 and Sc-ACS3 in the immature internodes were higher than in the mature internodes during sucrose accumulation. Ethephon significantly increased expressions of the three genes in the internodes stalk, which were in accordance of the significant effect on increasing the ethylene production and the sucrose % cane in the internodes. Significance of Study: 1-aminocyclopropane-1-carboxylic acid synthase is one of thekey rate-limiting enzymes for ethylene biosynthesis in sugarcane. The data in the present study provided references for understanding the relationship between the genges expressions and ethylene production and sucrose accumulation in sugarcane at molecular level.

分类号： TS24

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