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CLONING AND SEQUENCING OF triangle open-PYRROLINE-5-CARBOXYLATE SYNTHETASE GENE FROM SUGARCANE

文献类型： 会议论文

第一作者： CHENG-MEI HUANG

作者： CHENG-MEI HUANG 1 ; LI-TAO YANG; YANG-RUI Li; YUAN-WEN WEI; ZHI-NIAN DENG; YOU-QIANG PAN; WEI-LI LU;

作者机构： 1.Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, China, 530007

关键词： triangle open—pyrroline-5-carboxylate synthetase gen;sugarcan;clonin;sequencing

会议名称： Technologies to improve sugar productivity in developing countries

主办单位：

页码： 590-593

摘要： Aims: To clone and sequence triangle open'-pyrroline-5-carboxylate synthetase gene from sugarcane. Methods and results: Sugarcane variety ROC22 (Saccharum officinarum L. Hybrid) was used as the experimental material. Water stress treatment was performed for the 4 or 5-leafed plants with 15-25% polyethylene glycol (PEG) 6000 solutions. By homologous based cloning and RT-PCR technique, the cDNA fragment of 935bp in length was isolated. By using the program of BLAST on NCBI GenBank database, the nucleicacid sequence of the fragment presented a very high match with the P5CS genes from other plants. After alignment, the identities of the cloned fragment were about 80% with the P5CS genes from rice and bread wheat. Significance of Study:: triangle open'-pyrroline-5-carboxlate synthase (P5CS) is a bifunctional enzyme catalyzing the first two steps of proline biosynthesis in plants. Cloning and sequencing triangle open'-pyrroline-5-carboxylate synthetase gene is important for illustrating the mechanism ofdrought resistance regulated by proline in sugarcane.

分类号： TS24

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