文献类型： 外文期刊

作者： Yang Zhen ¹ ; Zhang Lan ² ; Zhao Jin-fa ¹ ; Zhang Xing-kai ¹ ; Wang Ying ¹ ; Li Tai-sheng ¹ ; Zhang Wei ³ ; Zhou Yan ¹ ;

作者机构： 1.Southwest Univ, Natl Citrus Engn & Technol Res Ctr, Citrus Res Inst, Chongqing 400712, Peoples R China

2.Guangxi Acad Agr Sci, Hort Res Inst, Nanning 530000, Peoples R China

3.Sichuan Agr & Rural Bur, Plant Protect Stn, Chengdu 610041, Peoples R China

关键词： Citrus chlorotic dwarf-associated virus; detection; sequence alignments; phylogenetic tree analysis

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.384； 五年影响因子：4.021 ）

ISSN： 2095-3119

年卷期： 2022 年 21 卷 1 期

页码：

收录情况： SCI

摘要： In 2009, an emerging citrus viral disease caused by Citrus chlorotic dwarf-associated virus (CCDaV) was discovered in Yunnan Province of China. However, the occurrence and spread of CCDaV in other citrus-growing provinces in China is unknown to date. To better understand the distribution and molecular diversity of CCDaV in China, a total of 1 772 citrus samples were collected from 11 major citrus-growing provinces and were tested for CCDaV by PCR. Among these, 134 citrus samples from Guangxi, Yunnan and Guangdong were tested positive for CCDaV, demonstrating that the occurrence and spread of CCDaV are increasing in China. The complete genome sequences of 17 CCDaV isolates from different provinces and hosts were sequenced. Comparisons of the whole-genome sequences of the 17 CCDaV isolates as well as the 15 isolates available in GenBank revealed that the sequence identity was about 99-100%, showing that the CCDaV isolates were highly conserved. Phylogenetic studies showed that the 32 CCDaV isolates belonged to four different groups based on geographical origins and host species, and that CCDaV isolates from China and Turkey were clustered into different groups. The results provide important information for clarifying the distribution and genetic diversity of CCDaV in China.