Selection of Reference Genes in Siraitia siamensis and Expression Patterns of Genes Involved in Mogrosides Biosynthesis
文献类型： 外文期刊
作者： Chen, Wenqiang 1 ; Lin, Xiaodong 1 ; Wang, Yan 1 ; Mu, Detian 1 ; Mo, Changming 2 ; Huang, Huaxue 3 ; Zhao, Huan 4 ; Luo, Zuliang 5 ; Liu, Dai 3 ; Wilson, Iain W. 6 ; Qiu, Deyou 7 ; Tang, Qi 1 ;
作者机构： 1.Hunan Agr Univ, Coll Hort, Yuelushan Lab, Changsha 410128, Peoples R China
2.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China
3.Hunan Huacheng Biotech Inc, Changsha 410205, Peoples R China
4.Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China
5.Chinese Acad Med Sci, Inst Med Plant Dev, Peking Union Med Coll, Beijing 100193, Peoples R China
6.CSIRO Agr & Food, Canberra, ACT 2601, Australia
7.Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China
关键词： Siraitia siamensis; reference genes; RT-qPCR; mogrosides; synthesis pathways
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 17 期
页码：
收录情况： SCI
摘要： Siraitia siamensis is a traditional Chinese medicinal herb. In this study, using S. siamensis cultivated in vitro, twelve candidate reference genes under various treatments were analyzed for their expression stability by using algorithms such as GeNorm, NormFinder, BestKeeper, Delta CT, and RefFinder. The selected reference genes were then used to characterize the gene expression of cucurbitadienol synthase, which is a rate-limiting enzyme for mogroside biosynthesis. The results showed that CDC6 and NCBP2 expression was the most stable across all treatments and are the best reference genes under the tested conditions. Utilizing the validated reference genes, we analyzed the expression profiles of genes related to the synthesis pathway of mogroside in S. siamensis in response to a range of abiotic stresses. The findings of this study provide clear standards for gene expression normalization in Siraitia plants and exploring the rationale behind differential gene expression related to mogroside synthesis pathways.
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