文献类型： 外文期刊

作者： Mu, Detian ¹ ; Shao, Yingying ¹ ; Zhao, Huan ³ ; Luo, Zuliang ² ; Mo, Changming ² ; Wilson, Iain W. ⁶ ; Pan, Limei ⁷ ; Huang, Huaxue ⁸ ; Chen, Wenqiang ¹ ; Lin, Xiaodong ¹ ; Zhou, Huimin ¹ ; Lu, Ying ¹ ; Qiu, Deyou ⁹ ; Ma, Xiaojun ³ ; Tang, Qi ¹ ;

作者机构： 1.Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Peoples R China

2.Yuelushan Lab, Changsha 410128, Peoples R China

3.Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China

4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100193, Peoples R China

5.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China

6.CSIRO Agr & Food, Canberra, ACT 2601, Australia

7.Guangxi Key Lab High Qual Format & Utilizat Dao Di, Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China

8.Hunan Huacheng Biotech Inc, Changsha 410205, Peoples R China

9.Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

关键词： Siraitia grosvenorii; genome-wide; mogroside V; SgTCP24; key enzyme genes

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 234 卷

页码：

收录情况： SCI

摘要： Siraitia grosvenorii, a important economic crop is now used as a sweetener because its bioactive compound, mogroside V, has high sweetness with zero calories. However, mogroside V only accumulates in S. grosvenorii fruits, and its content is low. Enhancing the production of mogroside V has become a major commercial and scientific endeavor. TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors (TFs) have a key role in regulating secondary metabolite biosynthesis, however, their role in regulating mogroside V is unclear. In this study, twenty-four SgTCP genes were identified based on genome-wide analysis and classified into two subgroups according to phylogenetic analysis. Based on the expression levels in different fruit development stages, co-expression network showed that SgTCP24 had a strong positive correlation with SgSQE, SgCS, and SgCYP87D18. Through the subcellular localization, yeast one hybrid, dual-luciferase, and electrophoretic mobility shift assay it was shown that SgTCP24 can directly bind and activate the expression of these three genes. When SgTCP24 was over-expressed in S. grosvenorii fruits, the production of mogroside V increased. Conversely, the content of mogroside V decreased in lines subjected to virus induced silencing. Our results provide a major insight into the molecular mechanism of TCP TFs in regulating the mogroside V biosynthesis, and offers a new method for enhancing the content of mogroside V in plants.