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Synthesis of Crocin I and Crocin II by Multigene Stacking in Nicotiana benthamiana

文献类型： 外文期刊

作者： Xie, Lei 1 ; Luo, Zuliang 1 ; Jia, Xunli 1 ; Mo, Changming 2 ; Huang, Xiyang 3 ; Suo, Yaran 4 ; Cui, Shengrong 1 ; Zang, Yimei 1 ; Liao, Jingjing 4 ; Ma, Xiaojun 1 ;

作者机构： 1.Chinese Acad Med Sci, Inst Med Plant Dev, Peking Union Med Coll, Beijing 100193, Peoples R China

2.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China

3.Guangxi Zhuang Autonomous Reg & Chinese Acad Sci, Guangxi Inst Bot, Guangxi Key Lab Funct Phytochem Res & Utilizat, Guilin 541006, Peoples R China

4.China Acad Chinese Med Sci, Artemisinin Res Ctr, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100700, Peoples R China

5.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

关键词： crocins; crocin I; crocin II; multigene assembly; plant chassis; synthetic biology

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 18 期

页码：

收录情况： SCI

摘要： Crocins are a group of highly valuable water-soluble carotenoids that are reported to have many pharmacological activities, such as anticancer properties, and the potential for treating neurodegenerative diseases including Alzheimer's disease. Crocins are mainly biosynthesized in the stigmas of food-medicine herbs Crocus sativus L. and Gardenia jasminoides fruits. The distribution is narrow in nature and deficient in resources, which are scarce and expensive. Recently, the synthesis of metabolites in the heterologous host has opened up the potential for large-scale and sustainable production of crocins, especially for the main active compounds crocin I and crocin II. In this study, GjCCD4a, GjALDH2C3, GjUGT74F8, and GjUGT94E13 from G. jasminoides fruits were expressed in Nicotiana benthamiana. The highest total content of crocins in T1 generation tobacco can reach 78,362 ng/g FW (fresh weight) and the dry weight is expected to reach 1,058,945 ng/g DW (dry weight). Surprisingly, the primary effective constituents crocin I and crocin II can account for 99% of the total crocins in transgenic plants. The strategy mentioned here provides an alternative platform for the scale-up production of crocin I and crocin II in tobacco.

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