Rapid colorimetric determination of the pesticides carbofuran and dichlorvos by exploiting their inhibitory effect on the aggregation of peroxidase-mimicking platinum nanoparticles
文献类型： 外文期刊
作者： Cao, Jing 2 ; Wang, Miao 1 ; She, Yongxin 1 ; Abd El-Aty, A. M. 3 ; Hacimuftuoglu, Ahmet 4 ; Wang, Jing 1 ; Yan, Mengm 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Standardizat & Testing Technol Agroprod, Beijing 100193, Peoples R China
2.Minist Agr, Key Lab Agrofood Safety & Qual Beijing, Beijing 100193, Peoples R China
3.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
4.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkey
5.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning 53003, Peoples R China
关键词： Organophosphates; Carbamates; Platinum nanoparticles; Enzyme inhibition; Colorimetric assay; Enzyme mimic
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
ISSN： 0026-3672
年卷期： 2019 年 186 卷 6 期
页码：
收录情况： SCI
摘要： A novel and highly sensitive enzyme inhibition assay was developed for the rapid detection of the organophosphate pesticide dichlorvos and the carbamate pesticide carbofuran. It achieves signal amplification by the secondary catalysis of platinum nanoparticles. Acetylcholinesterase (AChE) is capable of catalyzing the hydrolysis of acetylthiocholine to form thiocholine. Thiocholine causes the aggregation of citrate-capped platinum nanoparticles which then lose their peroxidase-mimicking properties. After addition of pesticides, the activity of AChE is inhibited, less thiocholine is produced, less aggregation occurs, and the peroxidase-mimetic properties are increasingly retained. In the presence of tetramethylbenzidine and H2O2, a deep blue coloration with an absorption maximum at 650 nm will be formed. The assay was applied to the determination of dichlorvos and carbofuran, and detection limits of 2.3 mu g.L-1 and 1.4 mu g.L-1 were obtained, respectively. Recovery experiments with spiked tap water and pears gave satisfactory relative standard deviations.
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