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A fluorescence aptamer sensor utilizing WS2 nanosheets for sensitive detection of patulin: enhanced specificity and wide applicability

文献类型： 外文期刊

作者： Qin, Guoxin 1 ; Li, Huiling 1 ; He, Jie 1 ; Wang, Haijun 1 ; Chen, Yongxian 1 ; Lao, Shuibing 1 ; Cheng, Liang 1 ; Lu, Weifan 1 ; Luo, Lihong 1 ; Tang, Li 1 ; Mo, Renfu 1 ; Wei, Yuning 1 ; Zhou, Qifeng 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Inst Agr Prod Qual Safety & Testing Technol, Nanning 530007, Peoples R China

期刊名称：ANALYTICAL METHODS （ 影响因子：2.7； 五年影响因子：2.7 ）

ISSN： 1759-9660

年卷期： 2024 年 16 卷 28 期

页码：

收录情况： SCI

摘要： A tungsten disulfide (WS2) nanosheet-based aptamer sensor was developed to detect patulin (PAT). The 5 '-end of the PAT aptamer was modified with a cyanine 3 (Cy3) fluorophore, which self-assembled on WS2 nanosheets. The interaction between the Cy3 fluorophore at the 5 '-end of the PAT aptamer and the WS2 nanosheets resulted in reduced fluorescence (FL) intensity due to fluorescence resonance energy transfer (FRET). The introduction of PAT into this sensing system led to hybridization with the PAT aptamer, forming a G-quadruplex/PAT complex with low affinity for the WS2 nanosheet surface. This hybridization increased the distance between the Cy3 fluorophore and the WS2 nanosheets, inhibiting FRET and producing a strong FL signal. Under optimal experimental conditions, the FL intensity of the sensing system demonstrated an excellent linear correlation with PAT concentrations ranging from 0.5 to 40.0 ng mL(-1), and it achieved a detection limit (S/N = 3) of 0.23 ng mL(-1). This sensing system offers enhanced specificity for PAT detection and has the potential for broad application in detecting other toxins by substituting the sequence of the recognition aptamer.

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