Mapping and validation of the epistatic D and P genes controlling anthocyanin biosynthesis in the peel of eggplant (Solanum melongena L.) fruit
文献类型： 外文期刊
作者： You, Qian 1 ; Li, Huimin 1 ; Wu, Jun 1 ; Li, Tao 1 ; Wang, Yikui 3 ; Sun, Guangwen 2 ; Li, Zhiliang 1 ; Sun, Baojuan 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Guangdong, Peoples R China
2.South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
3.Guangxi Acad Agr Sci, Inst Vegetable, Nanning 530007, Guangxi, Peoples R China
4.Gannan Acad Sci, Ganzhou Res Inst Vegetables & Flowers, Ganzhou 341400, Jiangxi, Peoples R China
期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）
ISSN： 2662-6810
年卷期： 2023 年 10 卷 2 期
页码：
收录情况： SCI
摘要： Fruit color is an important trait influencing the commercial value of eggplant fruits. Three dominant genes (D, P and Y) cooperatively control the anthocyanin coloration in eggplant fruits, but none has been mapped. In this study, two white-fruit accessions (19 141 and 19 147) and their F-2 progeny, with 9:7 segregation ratio of anthocyanin pigmented versus non-pigmented fruits, were used for mapping the D and P genes. A high-density genetic map was constructed with 5270 SNPs spanning 1997.98 cM. Three QTLs were identified, including two genes on chromosome 8 and one on chromosome 10. Gene expression analyses suggested that the SmANS on chromosome 8 and SmMYB1 on chromosome 10 were the putative candidate genes for P and D, respectively. We further identified (1) a SNP leading to a premature stop codon within the conserved PLN03176 domain of SmANS in 19 141, (2) a G base InDel in the promoter region leading to an additional cis-regulatory element and (3) a 6-bp InDel within the R2-MYB DNA binding domain of SmMYB1, in 19 147. Subsequently, these three variations were validated by PARMS technology as related to phenotypes in the F-2 population. Moreover, silencing of SmANS or SmMYB1 in the purple red fruits of F-1 (E3316) led to inhibition of anthocyanin biosynthesis in the peels. Conversely, overexpression of SmANS or SmMYB1 restored anthocyanin biosynthesis in the calli of 19 141 and 19 147 respectively. Our findings demonstrated the epistatic interactions underlying the white color of eggplant fruits, which can be potentially applied to breeding of eggplant fruit peel color.
- 相关文献
作者其他论文 更多>>
-
Understanding the nutraceutical diversity through a comparative analysis of the taproot metabolomes of different edible radish types via UHPLC-Q-TOF-MS
作者：Wang, Jinglei;Wei, Qingzhen;Wang, Wuhong;Hu, Haijiao;Yan, Yaqin;Hu, Tianhua;Bao, Chonglai;Wang, Yikui;Li, Yan;Jiang, Yaqin;Wu, Guanzheng
关键词：Radish; UHPLC-Q-TOF-MS; Metabolomics; Edible types
-
Fine Mapping and Candidate Gene Analysis of the Gv1 Locus Controlling Green-Peel Color in Eggplant (Solanum melongena L.)
作者：Lv, Zijian;Jin, Qingmin;Li, Zhiliang;Li, Tao;You, Qian;Gong, Chao;Heng, Zhou;Sun, Baojuan;Lv, Zijian;Wang, Yikui
关键词：eggplant; green peel color; Gv1 gene; SmAPPR2-like
-
Defensive Resistance of Cowpea Vigna unguiculata Control Megalurothrips usitatus Mediated by Jasmonic Acid or Insect Damage
作者：Li, Tao;Feng, Mingyue;Chi, Yuanming;Sun, Zilin;Shi, Wangpeng;Li, Tao;Feng, Mingyue;Chi, Yuanming;Sun, Zilin;Shi, Wangpeng;Shi, Xing;Wu, Zhen;Li, Aomei
关键词：cowpea; bean flower thrips; transcriptome; metabolome; induced resistance
-
The chromosome-scale reference genome and transcriptome analysis of Solanum torvum provides insights into resistance to root-knot nematodes
作者：Zhang, Hongyuan;Chen, Hao;Tan, Jie;Huang, Shuping;Chen, Xia;Dong, Hongxia;Wang, Benqi;Zhang, Min;Zhang, Ru;Hu, Jihong;Wang, Yikui;Xiao, Xueqiong;Hong, Zonglie;Zhang, Junhong
关键词：Solanum torvum; genome; transcriptome; root-knot nematode; ABC transporter
-
SlGH3.15, a member of the GH3 gene family, regulates lateral root development and gravitropism response by modulating auxin homeostasis in tomato
作者：Ai, Guo;Huang, Rong;Zhang, Dedi;Li, Miao;Li, Guobin;Li, Wangfang;Ahiakpa, John K.;Zhang, Junhong;Wang, Yikui;Hong, Zonglie
关键词：Auxin; Hormonal homeostasis; Solanum lycopersicum; Root formation; GH3 gene
-
A novel electrochemical aptasensor based on polyaniline and gold nanoparticles for ultrasensitive and selective detection of ascorbic acid
作者：Jiang, Cuiwen;Xie, Liping;Yan, Feiyan;Liang, Zhongdan;Liang, Jing;Li, Huiling;Wang, Yanli;Luo, Lihong;Li, Tao;Ning, Dejiao;Tang, Li;Ya, Yu;Huang, Kejing
关键词：
-
Characteristics, Comparative Analysis, and Phylogenetic Relationships of Chloroplast Genomes of Cultivars and Wild Relatives of Eggplant (Solanum melongena)
作者：Yang, Qihong;Cai, Liangyu;Gan, Guiyun;Wang, Peng;Li, Weiliu;Li, Wenjia;Jiang, Yaqin;Li, Dandan;Wang, Mila;Chen, Riyuan;Wang, Yikui;Li, Ye;Wang, Mila;Chen, Riyuan;Xiong, Cheng
关键词：Solanum melongena; chloroplast genome; DNA barcode; comparative analysis; phylogenetic; interspecific hybridization
