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Proteome-wide identification of non-histone lysine methylation in tomato during fruit ripening

文献类型： 外文期刊

作者： Xiao, Lu 1 ; Liang, Hanzhi 3 ; Jiang, Guoxiang 1 ; Ding, Xiaochun 1 ; Liu, Xuncheng 1 ; Sun, Jian 7 ; Jiang, Yueming 1 ; Song, Lili 1 ; Duan, Xuewu 1 ;

作者机构： 1.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China

2.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Guangzhou 510650, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Guangdong Acad Agr Sci, Inst Qual Stand & Monitoring Technol Agroprod, Guangzhou 510640, Peoples R China

5.Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Agroprod Safety & Qual, Guangzhou 510640, Peoples R China

6.Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Linan 311300, Zhejiang, Peoples R China

7.Guangxi Acad Agr Sci, Agrofood Sci & Technol Res Inst, Nanning 530007, Peoples R China

关键词： Non-histone; Lysine; Methylome; Post-translational modification; Tomato; Ripening

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：12.822； 五年影响因子：11.664 ）

ISSN： 2090-1232

年卷期： 2022 年 42 卷

页码：

收录情况： SCI

摘要： Introduction: Histone and non-histone methylations are important post-translational modifications in plants. Histone methylation plays a crucial role in regulating chromatin structure and gene expression. However, the involvement of non-histone methylation in plant biological processes remains largely unknown.Methods: The methylated substrates and methylation sites during tomato fruit ripening were identified by LC-MS/MS. Bioinformatics of lysine methylated proteins was conducted to analyze the possible role of methylated proteins. The effects of methylation modification on protein functions were preliminarily investigated by site-directed mutation simulation.Results: A total of 241 lysine methylation (mono-, di-and trimethylation) sites in 176 proteins were identified with two conserved methylation motifs: xxxxxxExxx_K_xxxExxxxxx and xxxxxxExxx_K_xxxxxxxxxx. These methylated proteins were mainly related to fruit ripening and senes-cence, oxidation reduction process, signal transduction, stimulus and stress responses, and energy meta-bolism. Three representative proteins, thioredoxin (Trx), glutathione S-transferase T1 (GST T1), and NADH dehydrogenase (NOX), were selected to investigate the effect of methylation modifications on pro-tein activity. Mimicking demethylation led to decreased Trx activity but increased GST T1 and NOX activ-ities. In addition, RT-qPCR exhibited that the expression of many genes that encode proteins subjected to methylation was upregulated during fruit ripening.Conclusion: Our study suggests that tomato fruit ripening undergo non-histone lysine methylation, which may participate in the regulation of fruit ripening. It is the first report of methyl proteome profiling of non-histone lysine in horticultural crops.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access articleunder the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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