文献类型： 外文期刊
作者： Liao, Jingjing 1 ; Liu, Tingyao 2 ; Xie, Lei 3 ; Mo, Changming 4 ; Huang, Xiyang 5 ; Cui, Shengrong 3 ; Jia, Xunli 3 ; Lan, Fusheng 6 ; Luo, Zuliang 3 ; Ma, Xiaojun 3 ;
作者机构： 1.China Acad Chinese Med Sci, Artemisinin Res Ctr, Inst Chinese Mat Med, Beijing 100700, Peoples R China
2.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China
3.Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China
4.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China
5.Guangxi Zhuang Autonomous Reg & Chinese Acad Sci, Guangxi Inst Bot, Guangxi Key Lab Plant Funct Phytochem & Sustainab, Guilin 541006, Peoples R China
6.Guilin GFS Monk Fruit Corp, Guilin 541006, Peoples R China
关键词： mogrosides; natural sweetener; plant chassis; multigene assembly; synthetic biology
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 18 期
页码：
收录情况： SCI
摘要： Mogrosides are a group of health-promoting natural products that extracted from Siraitia grosvenorii fruit (Luo-han-guo or monk fruit), which exhibited a promising practical application in natural sweeteners and pharmaceutical development. However, the production of mogrosides is inadequate to meet the need worldwide, and uneconomical synthetic chemistry methods are not generally recommended for structural complexity. To address this issue, an in-fusion based gene stacking strategy (IGS) for multigene stacking has been developed to assemble 6 mogrosides synthase genes in pCAMBIA1300. Metabolic engineering of Nicotiana benthamiana and Arabidopsis thaliana to produce mogrosides from 2,3-oxidosqualene was carried out. Moreover, a validated HPLC-MS/MS method was used for the quantitative analysis of mogrosides in transgenic plants. Herein, engineered Arabidopsis thaliana produced siamenoside I ranging from 29.65 to 1036.96 ng/g FW, and the content of mogroside III at 202.75 ng/g FW, respectively. The production of mogroside III was from 148.30 to 252.73 ng/g FW, and mogroside II-E with concentration between 339.27 and 5663.55 ng/g FW in the engineered tobacco, respectively. This study provides information potentially applicable to develop a powerful and green toolkit for the production of mogrosides.
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