文献类型： 外文期刊
作者： Liao, Jingjing 1 ; Liu, Tingyao 2 ; Xie, Lei 3 ; Mo, Changming 4 ; Huang, Xiyang 5 ; Cui, Shengrong 3 ; Jia, Xunli 3 ; Lan, Fusheng 6 ; Luo, Zuliang 3 ; Ma, Xiaojun 3 ;
作者机构： 1.China Acad Chinese Med Sci, Artemisinin Res Ctr, Inst Chinese Mat Med, Beijing 100700, Peoples R China
2.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China
3.Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China
4.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China
5.Guangxi Zhuang Autonomous Reg & Chinese Acad Sci, Guangxi Inst Bot, Guangxi Key Lab Plant Funct Phytochem & Sustainab, Guilin 541006, Peoples R China
6.Guilin GFS Monk Fruit Corp, Guilin 541006, Peoples R China
关键词： mogrosides; natural sweetener; plant chassis; multigene assembly; synthetic biology
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 18 期
页码：
收录情况： SCI
摘要： Mogrosides are a group of health-promoting natural products that extracted from Siraitia grosvenorii fruit (Luo-han-guo or monk fruit), which exhibited a promising practical application in natural sweeteners and pharmaceutical development. However, the production of mogrosides is inadequate to meet the need worldwide, and uneconomical synthetic chemistry methods are not generally recommended for structural complexity. To address this issue, an in-fusion based gene stacking strategy (IGS) for multigene stacking has been developed to assemble 6 mogrosides synthase genes in pCAMBIA1300. Metabolic engineering of Nicotiana benthamiana and Arabidopsis thaliana to produce mogrosides from 2,3-oxidosqualene was carried out. Moreover, a validated HPLC-MS/MS method was used for the quantitative analysis of mogrosides in transgenic plants. Herein, engineered Arabidopsis thaliana produced siamenoside I ranging from 29.65 to 1036.96 ng/g FW, and the content of mogroside III at 202.75 ng/g FW, respectively. The production of mogroside III was from 148.30 to 252.73 ng/g FW, and mogroside II-E with concentration between 339.27 and 5663.55 ng/g FW in the engineered tobacco, respectively. This study provides information potentially applicable to develop a powerful and green toolkit for the production of mogrosides.
- 相关文献
作者其他论文 更多>>
-
Effect of foliar application of Siraitia grosvenorii-derived carbon dots on growth, photosynthesis, and physiological characteristics of Siraitia grosvenorii
作者：Zheng, Yumei;Tang, Hui;Yuan, Yuan;Wu, Mengying;Huang, Xiyang;Zhang, Lizhen;Qin, Xinmei;Wei, Rongchang
关键词：Carbon dots; Crop waste utilization; Photosynthesis; Plant physiology; Siraitia grosvenorii
-
Genome-Wide Identification and Characterization of AGO, DCL, and RDR Gene Families in Siraitia grosvenorii
作者：Zang, Yimei;Wang, Chongnan;Su, Jiaxian;Xie, Lei;Luo, Zuliang;Ma, Xiaojun;Mo, Changming;Mo, Changming
关键词：
Siraitia grosvenorii ; Argonaute (AGO); Dicer-like (DCL); RNA-dependent RNA polymerase (RDR) -
A novel transcription factor SgTCP24 positively regulates mogroside V biosynthesis by activating SgSQE, SgCS, and SgCYP87D18 expression in Siraitia grosvenorii
作者：Mu, Detian;Shao, Yingying;Chen, Wenqiang;Lin, Xiaodong;Zhou, Huimin;Lu, Ying;Tang, Qi;Mu, Detian;Shao, Yingying;Luo, Zuliang;Mo, Changming;Lu, Ying;Tang, Qi;Zhao, Huan;Ma, Xiaojun;Luo, Zuliang;Mo, Changming;Wilson, Iain W.;Pan, Limei;Huang, Huaxue;Qiu, Deyou
关键词：Siraitia grosvenorii; genome-wide; mogroside V; SgTCP24; key enzyme genes
-
Selection of Reference Genes in Siraitia siamensis and Expression Patterns of Genes Involved in Mogrosides Biosynthesis
作者：Chen, Wenqiang;Lin, Xiaodong;Wang, Yan;Mu, Detian;Tang, Qi;Mo, Changming;Huang, Huaxue;Liu, Dai;Zhao, Huan;Luo, Zuliang;Wilson, Iain W.;Qiu, Deyou
关键词：Siraitia siamensis; reference genes; RT-qPCR; mogrosides; synthesis pathways
-
Regulatory Effects of Chlormequat Chloride on the Yield and Chemical Composition of Angelica sinensis Radix
作者：Qin, Honghan;Qin, Honghan;Xu, Juan;Ma, Xiaojun;Luo, Zuliang;Wei, Rongchang
关键词：Angelica sinensis; chlormequat chloride; regulatory effects; chemical composition
-
Synthesis of Crocin I and Crocin II by Multigene Stacking in Nicotiana benthamiana
作者：Xie, Lei;Luo, Zuliang;Jia, Xunli;Cui, Shengrong;Zang, Yimei;Ma, Xiaojun;Mo, Changming;Huang, Xiyang;Suo, Yaran;Liao, Jingjing;Suo, Yaran;Liao, Jingjing
关键词：crocins; crocin I; crocin II; multigene assembly; plant chassis; synthetic biology
-
Post-Ripening and Key Glycosyltransferase Catalysis to Promote Sweet Mogrosides Accumulation of Siraitia grosvenorii Fruits
作者：Cui, Shengrong;Zang, Yimei;Xie, Lei;Su, Jiaxian;Jia, Xunli;Luo, Zuliang;Ma, Xiaojun;Zang, Yimei;Mo, Changming
关键词：Siraitia grosvenorii; mogrosides; metabolomics; post-ripening; UGT94-289-3
