文献类型： 外文期刊
作者： Liao, Jingjing 1 ; Xie, Lei 1 ; Liu, Tingyao 2 ; Mo, Changming 3 ; Cui, Shengrong 1 ; Jia, Xunli 4 ; Huang, Xiyang 5 ; Luo, Zuliang 1 ; Ma, Xiaojun 1 ;
作者机构： 1.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China
2.Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China
3.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China
4.Chinese Acad Med Sci, Yunnan Branch, Inst Med Plant Dev, Yunnan Key Lab Southern Med Utilizat, Jinghong 666100, Peoples R China
5.Guangxi Zhuang Autonomous Reg & Chinese Acad Sci, Guangxi Inst Bot, Guangxi Key Lab Plant Funct Phytochem & Sustainab, Guilin 710089, Peoples R China
关键词： baicalein; multigene vector; Lycopersicon esculentum; plant chassis; biofortification; heterologous biosynthesis
期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）
ISSN：
年卷期： 2022 年 27 卷 10 期
页码：
收录情况： SCI
摘要： Baicalein is a valuable flavonoid isolated from the medicinal plant Scutellaria baicalensis Georgi, which exhibits intensive biological activities, such as anticancer and antiviral activities. However, its production is limited in the root with low yield. In this study, In-Fusion and 2A peptide linker were developed to assemble SbCLL-7, SbCHI, SbCHS-2, SbFNSII-2 and SbCYP82D1.1 genes driven by the AtPD7, CaMV 35S and AtUBQ10 promoters with HSP, E9 and NOS terminators, and were used to engineer baicalein biosynthesis in transgenic tomato plants. The genetically modified tomato plants with this construct synthesized baicalein, ranging from 150 ng/g to 558 ng/g FW (fresh weight). Baicalein-fortified tomatoes have the potential to be health-promoting fresh vegetables and provide an alternative source of baicalein production, with great prospects for market application.
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