A high throughput method for rapid screening of chitosanase-producing fungal strain under acidic conditions
文献类型： 外文期刊
作者： Ding, Su 1 ; Chen, Gui-Guang 1 ; Liang, Zhi-Qun 1 ; Zeng, Wei 1 ; Cao, Mu-Ming 4 ; Chen, Guo-Pin; Xie, Shu-Yu; Li, 1 ;
作者机构： 1.Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Guangxi, Peoples R China
2.Guangxi Univ, Key Lab, Minist Educ Microbial & Plant Genet Engn, Nanning 530004, Guangxi, Peoples R China
3.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Guangxi, Peoples R China
4.Guangxi Acad Agr Sci, Viticulture & Wine Res Inst, Nanning
关键词： High-throughput method;Chitooligosaccharides;Chitosanase;Aspergillus fumigatus
期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： A novel high-throughput method was established for rapid screening of a large numbers of Aspergillus fumigatus ( A. fumigatus) mutants with high chitosanase production under acidic culture condition by exploiting the fact that iodine can be used as the indicator to stain chitosan but is ineffective for chitooligosaccharides. The mutant population was generated by irradiating A. fumigatus CICC 2434 with Co-60-gamma rays. Mutants were cultured on acidic plates containing colloidal chitosan and preliminary screened according to diameter of haloes formed around colonies. Then, chitosanase production of the isolates were verified by dinitrosalicylic acid assay. Lastly, molecular masses on enzymolysis products of isolated mutants were rapidly compared by aniline blue plate assay. Using this method, the mutant strain Co-8 was selected, which had chitosanase activity of 24.87 U/mL ( increased by 369.2 % as compared to that of its parental strain). Taking together, the method is easy, efficient and particularly suited to rapid screen acidophilic fungal strains with high chitosanase-production.
- 相关文献
作者其他论文 更多>>
-
Identification and characterization of Colletotrichum species associated with anthracnose disease of passion fruit in Guangxi, China
作者：Zhao, Sifan;Huang, Suiping;Tang, Lihua;Chen, Xiaolin;Zhang, Yu;Guo, Tangxun;Li, Qili;Zhao, Sifan;Li, Wei;Sun, Wenxiu;Hsiang, Tom
关键词：
Colletotrichum ; morphological characteristics; passion fruit; pathogenicity; phylogenetic analysis; analyse phylog & eacute;n & eacute;tique; caract & eacute;ristiques morphologiques; fruit de la passion; pathog & eacute;nicit & eacute; -
Modulating 11S/7S ratios in lactic acid bacteria-fermented soy protein gels: effects on physicochemical properties and allergenicity
作者：Lu, Xiaocui;Yao, Linjing;Li, Wei;Rui, Xin;He, Jie;Wang, Haijun
关键词：11S/7S ratio; lactic acid bacteria fermentation; IgE reactivity; gelation
-
Compositional Shifts in the Mycobiota of 'Shine Muscat' Grape (Vitis labruscana Baily x V. vinifera L.) Bunches During Cold Storage at Different Temperatures
作者：Li, Wei;Xie, Linjun;Zhou, Yongmei;Pang, Liting;Liang, Feicui;Cheng, Guo;Zhang, Jin;Li, Wei;Wang, Haijun;Cheng, Guo;Zhang, Jin;Ji, Xian;Ji, Xian
关键词：
Vitis ; fungal diversity; high-throughput sequencing; post-harvest -
Metabolome and transcriptome analyses provide insight into the effect of 1-MCP and SO 2 preservatives on the synthesis and regulation of phenols in 'Shine Muscat ' storage grapes
作者：Zhang, Jin;Xie, Linjun;Zhou, Sihong;Xie, Taili;Zhou, Yongmei;Li, Wei;Pang, Liting;Cheng, Guo;Zhang, Jin;Sun, Jian;Cheng, Guo;Zhang, Jin;Xie, Linjun;Wang, Haijun;Sun, Jian;Cheng, Guo;Zhou, Sihong;Zhu, Zhiqiang
关键词：Chemical preservatives; 'Shine Muscat' grape; Phenolic metabolism; Metabolomics; Transcriptomics
-
Transcriptome Analysis Reveals the Molecular Mechanism of Grape Inflorescence Elongation after Applying Microbial Fertilizers
作者：Huang, Qiufeng;Chen, Guopin;Xie, Shuyu;Li, Wei;Huang, Jing;Huang, Yu;Guan, Jingxi;Cao, Muming
关键词：dynamic transcriptomic; gibberellic acid; phytohormone signal; transcription factors; Vitis vinifera
-
Solid-state fermentation alters the fate of red kidney bean protein during buccal and gastrointestinal digestion: Relationship with cotyledon cell wall integrity
作者：Sun, Wenjing;Zhang, Qiuqin;Li, Wei;Rui, Xin;He, Jie;Wang, Haijun;Rui, Xin;Rui, Xin
关键词：Red kidney bean; Protein digestibility; Solid-state fermentation; Cotyledon cell; Buccal digestion; Gastrointestinal digestion
-
Improving the thermostability of alginate lyase FlAlyA with high expression by computer-aided rational design for industrial preparation of alginate oligosaccharides
作者：Zhang, Xiu;Zhang, Yunkai;Zhang, Xiu;Pan, Lixia;Yang, Liyan;Li, Hongliang;Yang, Dengfeng;Li, Wei;Ji, Feng;Tang, Hongzhen
关键词：alginate lyase; high-level expression; thermostability; rational design; molecular dynamics simulation; alginate oligosaccharide
