Determination of Dichlorvos in Pears by Surface-Enhanced Raman Scattering (SERS) with Catalysis by Platinum Coated Gold Nanoparticles
文献类型： 外文期刊
作者： Yu, He 1 ; Wang, Miao 1 ; Cao, Jing 1 ; She, Yongxin 1 ; Zhu, Yongan 1 ; Ye, Jiaming 3 ; Wang, Jing 1 ; Lao, Shuibing 4 ; Ab 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Minist Agr, Key Lab Agrofood Safety & Qual Beijing, Beijing, Peoples R China
3.Tsinghua Univ, Yangtze Delta Reg Inst, Jiaxing, Peoples R China
4.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning, Peoples R China
5.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza, Egypt
6.Ataturk Univ, Med Fac, Dept Med Pharmacol, Erzurum, Turkey
关键词： Acetylcholinesterase (AChE); dichlorvos; organophosphate pesticides; platinum coated gold nanoparticles; surface-enhanced Raman scattering (SERS)
期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）
ISSN： 0003-2719
年卷期：
页码：
收录情况： SCI
摘要： A surface-enhanced Raman scattering (SERS) sensor is reported for the determination of dichlorvos, an organophosphate pesticide (OP), in pears. Platinum coated gold nanoparticles (Au@PtNPs) were used as secondary catalysts to provide signal amplification. Acetylcholinesterase (AChE) catalyzed the hydrolysis of acetylthiocholine to form thiocholine. Thiocholine causes the accumulation of Au@PtNPs, which changes the particle size and prevents their peroxidase-mimicking activity. Dynamic light scattering was used to characterize the particle size. Following the addition of dichlorvos, the AChE activity was inhibited, the production of thiocholine and aggregation were reduced, and the peroxidase mimicing property was retained. In the presence of 3,3 ',5,5 '-tetramethylbenzidine (TMB), a blue product was produced that provided strong SERS signals. The SERS method showed a linear relationship for dichlorvos concentrations from 20 to 2000 mu g/L. The developed protocol had a limit of detection of 20 mu g/L. This SERS sensor assay was successfully applied for the quantification of dichlorvos in pears with satisfactory recoveries from 80.25 to 94.86%.
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