Validation and Application of a Molecular Detection System for Fusarium Wilt of Banana in China
文献类型： 外文期刊
作者： Yang, Di 1 ; Du, Chanjuan 1 ; Zhang, Jin 1 ; Pan, Lianfu 1 ; Wei, Shaolong 1 ; Jiang, Shangbo 1 ; Li, Chunyu 2 ; San, Cho Cho 3 ; Huy, Nguyen Duc 4 ; Ye, Yunfeng 5 ; Fu, Gang 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Plant Protect Res Inst, Key Lab Green Prevent & Control Fruits & Vegetable, Minist Agr & Rural Affairs,Guangxi Key Lab Biol Cr, Nanning 530007, Peoples R China
2.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Peoples R China
3.Dept Agr Res, Plant Pathol Res Sect, Naypyitaw 15013, Myanmar
4.Vietnam Natl Univ Agr, Fac Agron, Dept Plant Pathol, Hanoi 100803, Vietnam
5.Guangxi Acad Agr Sci, Hort Res Inst, Nanning 530007, Peoples R China
关键词： Fusarium oxysporum f. sp. cubense; molecular detection; primer evaluation
期刊名称：PLANT DISEASE （ 影响因子：4.5； 五年影响因子：5.0 ）
ISSN： 0191-2917
年卷期： 2023 年 107 卷 12 期
页码：
收录情况： SCI
摘要： Fusarium wilt of banana is a devastating disease caused by Fusarium oxysporum f. sp. cubense (Foc). It has restricted the development of the banana industry worldwide and is particularly serious in China because of the large planting areas and special planting patterns. However, there is no rapid and accurate approach to detect the Foc strains that specifically occur in China because of the rich genetic diversity observed in this pathosystem. In this study, we evaluated the performance of 10 previously published PCR primer pairs on 103 representative Foc strains in China and neighboring countries and screened out a set of primers (Foc-specific primer pair SIX9-Foc-F/R, Foc R1-specific primer pair SIX6b-210-F/R, Foc R4-specific primer pair Foc-1/2, and Foc TR4-specific primer pair W2987F/R) suitable for the detection of Foc strains in China and the surrounding Southeast Asian countries. Moreover, we developed a molecular detection system to accurately identify the different physiological races of Foc. The findings of this study provide technical support for preventing and controlling the spread of Fusarium wilt of banana in the field in China.
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