The inhibitory effect of citrus flavonoids naringenin and hesperetin against purine nucleoside phosphorylase: Spectroscopic, atomic force microscopy and molecular modeling studies
文献类型： 外文期刊
作者： Lv, Xingang 1 ; Wang, Qilei 1 ; Wang, Lang-Hong 1 ; Ren, Er-Fang 2 ; Gong, Deming 3 ;
作者机构： 1.Northwest Univ, Coll Food Sci & Technol, Xian 710069, Peoples R China
2.Guangxi Subtrop Crops Res Inst, Nanning 530001, Peoples R China
3.New Zealand Inst Nat Med Res, Dept Biomed, Auckland 2104, New Zealand
关键词： Citrus flavonoid; Purine nucleoside phosphorylase; Enzymatic kinetics; Molecular modeling; Atomic force microscopy
期刊名称：JOURNAL OF MOLECULAR LIQUIDS （ 影响因子：6.165； 五年影响因子：5.642 ）
ISSN： 0167-7322
年卷期： 2020 年 307 卷
页码：
收录情况： SCI
摘要： In this work, the inhibitory effect of two citrus flavonoids naringenin and hesperetin on human purine nucleoside phosphorylase (hPNP) and their binding mechanism were evaluated. Results from enzymatic kinetics revealed that naringenin and hesperetin reversibly inhibited hPNP via a mixed-type manner with IC50 values of 4.83 x 10(-4) M and 5.32 x 10(-4) M, respectively. Analysis of molecular modeling revealed that both naringenin and hesperetin bound directly into the active site by generating multiple forces including hydrogen bonding, p-p and p-Alkyl interactions with His64, Glu201, Ser220, His257, Phe200 and Val217 residues of hPNP, which caused the inhibition of hPNP activity. Moreover, conformational analysis by three-dimension fluorescence, circular dichroism and atomic force microscopy revealed that the binding of naringenin and hesperetin to hPNP induced changes in the microenvironment, secondary structure and morphology of hPNP. These results suggested that occupying the active site and enzymatic conformational perturbation induced by naringenin and hesperetin are the main reasons for reducing the inhibition of hPNP activity, which would be helpful in understanding the inhibitory mechanism of naringenin and hesperetin against hPNP. (C) 2020 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Insight into the mechanism of efficient extraction of tea saponins from Camellia oleifera shells using deep eutectic solvents
作者：Mo, Xiangyan;Hang, Fangxue;Ren, Er-Fang;Gai, Lili;Li, Kai;Niu, Debao;Ren, Er-Fang
关键词：Camellia oleifera shells; Deep eutectic solvent; Tea saponins; UPLC-Q-Orbitrap HRMS; Molecular dynamic simulation; Extraction mechanism
-
Revealing the multi-target destruction induced by proanthocyanidins against Acetobacter sp. at the molecular level
作者：Ren, Er-Fang;Feng, Xiaoqin;Feng, Yuanxin;Li, Kai;Chen, Shan;Niu, Debao;Ren, Er-Fang;Zeng, Xin-An;Cai, Jin-Lin;Han, Zhong;Wen, Qing-Hui
关键词：proanthocyanidins; cell membrane; membrane fatty acid; enzyme activity; genomic DNA; molecular docking
-
Application of Microbial Fermentation in Caffeine Degradation and Flavor Modulation of Coffee Beans
作者：Ran, Lu-Xia;Wei, Xiang-Ying;Ren, Er-Fang;Qin, Jian-Feng;Rasheed, Usman;Chen, Gan-Lin;Ran, Lu-Xia;Wei, Xiang-Ying;Rasheed, Usman;Chen, Gan-Lin;Ran, Lu-Xia;Wei, Xiang-Ying;Rasheed, Usman;Chen, Gan-Lin
关键词：decaffeination; low-caffeine coffee; microbial fermentation; mixed-culture; flavor enhancement
-
Characterization of the Pure Black Tea Wine Fermentation Process by Electronic Nose and Tongue-Based Techniques with Nutritional Characteristics
作者：Chen, Jing;Lin, Bo;Zheng, Feng-Jin;Fang, Xiao-Chun;Wu, Fei-Fei;Chen, Gan-Lin;Lin, Bo;Zheng, Feng-Jin;Fang, Xiao-Chun;Chen, Gan-Lin;Chen, Gan-Lin;Chen, Jing;Wu, Fei-Fei;Ren, Er-Fang;Verma, Krishan K.
关键词：
-
Ultrasonic-Assisted Dual-Alkali Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse Followed by Candida tropicalis Fermentation to Produce Xylitol
作者：Gai, Lili;Tian, Wen;Niu, Debao;Sun, Weidong;Hang, Fangxue;Li, Kai;Ren, Er-Fang
关键词：ultrasonic; enzymatic hydrolysis; sugarcane bagasse; xylitol; pretreatment
-
Review of the application of pulsed electric fields (PEF) technology for food processing in China
作者：Niu, Debao;Zeng, Xin-An;Xu, Fei-Yue;Li, Jian;Wang, Rui;Niu, Debao;Zeng, Xin-An;Xu, Fei-Yue;Li, Jian;Wang, Rui;Ren, Er-Fang;Wang, Man-Sheng
关键词：Pulsed electric fields (PEF); Extraction; Modification; Chemical reaction; Food processing
-
Multi-target antibacterial mechanism of eugenol and its combined inactivation with pulsed electric fields in a hurdle strategy on Escherichia coli
作者：Niu, Debao;Wang, Qian-Yi;Zeng, Xin-An;Wang, Lang-Hong;He, Tian-Fu;Wen, Qing-Hui;Niu, Debao;Wang, Qian-Yi;Zeng, Xin-An;Wang, Lang-Hong;He, Tian-Fu;Wen, Qing-Hui;Brennan, Charles S.;Ren, Er-Fang;Brennan, Charles S.;Brennan, Charles S.
关键词：Eugenol; Escherichia coli; Membrane integrity; DNA binding; Combined effect
