An Overview on the Mechanisms and Applications of Enzyme Inhibition-Based Methods for Determination of Organophosphate and Carbamate Pesticides
文献类型： 外文期刊
作者： Cao, Jing 1 ; Wang, Miao 1 ; Yu, He 1 ; She, Yongxin 1 ; Cao, Zhen 1 ; Ye, Jiaming 4 ; Abd El-Aty, A. M. 5 ; Hacimuftuogl 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Standardizat & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Minist Agr, Key Lab Agrofood Safety & Qual Beijing, Beijing 100193, Peoples R China
3.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res Inst, Nanning 53003, Peoples R China
4.Tsinghua Univ, Yangtze Delta Reg Inst, Jiaxing 314006, Peoples R China
5.Qilu Univ Technol, Shandong Acad Sci, Coll Food Sci & Engn, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Peoples R China
6.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
7.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkey
关键词： organophosphate pesticides; carbamate pesticides; rapid detection methods; devices; enzyme sources; reactivation; identification
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2020 年 68 卷 28 期
页码：
收录情况： SCI
摘要： Acetylcholinesterase inactivating compounds, such as organophosphate (OP) and carbamate (CM) pesticides, are widely used in agriculture to ensure sustainable production of food and feed. As a consequence of their applications, they would result in neurotoxicity, even death. In this essence, the development of enzyme inhibition methods still shows great significance as rapid detection techniques for on-site large-scale screening of OPs and CMs. Initially, mechanisms and applications of various enzyme-inhibition-based methods and devices, including optical colorimetric assay, fluorometric assays, electrochemical biosensors, rapid test card, and microfluidic device, are highlighted in the present overview. Further, to enhance the enzyme sensitivity for detection; alternative enzyme sources or high yield enrichment methods (such as abzyme, artificial enzyme, and recombinant enzyme), as well as enzyme reactivation and identification, are also addressed in this comprehensive overview.
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