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Comparative transcriptome analysis provides insights into the molecular mechanism underlying double fertilization between self-crossedSolanum melongenaand that hybridized withSolanum aethiopicum

文献类型： 外文期刊

作者： Li, Dandan 1 ; Li, Siqi 1 ; Li, Wenjia 2 ; Liu, Ake 2 ; Jiang, Yaqin 2 ; Gan, Guiyun 2 ; Li, Weiliu 2 ; Liang, Xuyu 1 ; Yu, 1 ;

作者机构： 1.South China Agr Univ, Coll Hort, Guangzhou, Peoples R China

2.Guangxi Acad Agr Sci, Inst Vegetable Res, Nanning, Peoples R China

3.Guangxi Key Lab Vegetable Breeding & New Technol, Nanning, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2020 年 15 卷 8 期

页码：

收录情况： SCI

摘要： Wild relatives represent a source of variation for many traits of interest for eggplant (Solanum melongena) breeding, as well as for broadening its genetic base. However, interspecific hybridization with wild relatives has been barely used in eggplant breeding programs, and reproductive barriers have resulted in reduced seed numbers in interspecific combinations. The mechanism underlying this phenomenon remains unclear. We hybridized females of cultivated eggplant 177 (Solanum melongena) with males of wild relatives 53 and Y11 (Solanum aethiopicum). Self-crossed 177 was the control. The seed number per control fruit was significantly higher than that of the hybrids. Paraffin sections showed no significant difference between control and 177x53 and 177xY11. Double fertilization began 4 days post-pollination. Sperm cells were fused with egg cells 6 days post-pollination. To understand the differences in molecular mechanisms underlying this process, transcriptomes of ovaries at0, 4, and 6 daysafter self-crossing and hybridization were analyzed. We screened22,311differentially expressed genes (DEGs) between the control and hybrids 4 and 6 days post-pollination. A total of 497 DEGs were shared among all pollination combinations. These DEGs were enriched in plant hormone transduction, cell senescence, metabolism, and biosynthesis pathways. DEG clustering analysis indicated distinct expression patterns between the control and hybrids but not between the hybrids. The DEGs in hybrids involved secondary metabolic process, phenylpropanoid metabolic process, and carboxypeptidase activity, while those in the control involved xyloglucan metabolic process, auxin-activated signaling pathway, cell wall polysaccharide metabolic process, and xyloglucosyl transferase activity. Additionally, 1683 transcription factors, including members of the AP2-ERF, MYB, bHLH, and B3 families may play important roles in self-crossing and hybridization. Our results provide insights into the regulatory mechanisms underlying variations between ovaries of self-crossed and hybrid eggplants and a basis for future studies on crossbreedingSolanumand genetic mechanisms underlying double fertilization.

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