Transcriptome Profiling Provides Molecular Insights into Auxin-Induced Adventitious Root Formation in Sugarcane (Saccharum spp. Interspecific Hybrids) Microshoots
文献类型： 外文期刊
作者： Li, Aomei 1 ; Lakshmanan, Prakash 1 ; He, Weizhong 1 ; Tan, Hongwei 1 ; Liu, Limin 1 ; Liu, Hongjian 1 ; Liu, Junxian; 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Sugarcane Res Inst, Key Lab Sugarcane Biotechnol & Genet Improvement, Minist Agr & Rural Affairs,Guangxi Key Lab Sugarc, Nanning 530007, Peoples R China
2.Southwest Univ, Coll Resources & Environm, Interdisciplinary Res Ctr Agr Green Dev Yangtze R, Chongqing 400715, Peoples R China
3.Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld 4072, Australia
关键词： sugarcane; auxin; transcriptomics; adventitious root formation
期刊名称：PLANTS-BASEL （ 影响因子：3.935； ）
ISSN：
年卷期： 2020 年 9 卷 8 期
页码：
收录情况： SCI
摘要： Adventitious root (AR) formation was enhanced following the treatment of sugarcane microshoots with indole-3-butyric acid (IBA) and 1-naphthalene acetic acid (NAA) combined, suggesting that auxin is a positive regulator of sugarcane microshoot AR formation. The transcriptome profile identified 1737 and 1268 differentially expressed genes (DEGs) in the basal tissues (5 mm) of sugarcane microshoots treated with IBA+NAA compared to nontreated control on the 3rd and 7th days post-auxin or water treatment (days post-treatment-dpt), respectively. To understand the molecular changes, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. This analysis showed that DEGs associated with the pathways were associated with plant hormone signaling, flavonoid and phenylpropanoid biosyntheses, cell cycle, and cell wall modification, and transcription factors could be involved in sugarcane microshoot AR formation. Furthermore, qRT-PCR analysis was used to validate the expression patterns of nine genes associated with root formation and growth, and the results were consistent with the RNA-seq results. Finally, a hypothetical hormonal regulatory working model of sugarcane microshoot AR formation is proposed. Our results provide valuable insights into the molecular processes associated with auxin-induced AR formation in sugarcane.
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