Dual-mode detection of organophosphate pesticides in pear and Chinese cabbage based on fluorescence and AuNPs colorimetric assays
文献类型： 外文期刊
作者： Yu, He 1 ; Wang, Miao 1 ; Cao, Jing 1 ; She, Yongxin 1 ; Zhu, Yongan 1 ; Ye, Jiaming 3 ; Abd El-Aty, A. M. 4 ; Hacimuftuo 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Minist Agr, Key Lab Agrofood Safety & Qual Beijing, Beijing 100081, Peoples R China
3.Tsinghua Univ, Yangtze Delta Reg Inst, Jiaxing 314006, Peoples R China
4.Qilu Univ Technol, Shandong Acad Sci, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Peoples R China
5.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
6.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkey
7.Guangxi Acad Agr Sci, Agroprod Qual Safety & Testing Technol Res In, Nanning 53003, Peoples R China
关键词： Fluorescence; Colorimetric; Dual-mode detection; Organophosphate pesticides; Residue detection; Enzyme inhibition
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2021 年 364 卷
页码：
收录情况： SCI
摘要： Herein, a dual-mode method based on fluorescent and colorimetric sensor was developed for determination of organophosphate pesticides (OPs). In this study, indoxyl acetate (IDA) was hydrolyzed by esterase into indophenol. Indophenol leads to changes in fluorescence signal and aggregation of gold nanoparticles (AuNPs); ultimately changing the color from red to blue. When OPs exist, the formation of indophenol was inhibited. With increasing the concentrations of OPs, the enhancement rate of fluorescence signal decreases, and the color change of AuNPs weakened gradually. The assay was applied for determination of dichlorvos, trichlorfon, and paraoxon, and the limits of detection (LODs) were 0.0032 mg/kg, 0.0096 mg/kg, and 0.0074 mg/kg (fluorometric assay), and 0.0120 mg/kg, 0.0224 mg/kg, and 0.0106 mg/kg (colorimetric assay), respectively. Finally, such a convenient and sensitive sensing assay was successfully applied for quantification of OPs in pear and Chinese cabbage with good recoveries ranged between 80.19 and 116.93%.
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